Explore the Fully-Characterized AAV Reference Standards (Full Capsids)
Serotype specific AAV reference standards are produced using helper virus-free method, triple transient transfection. Full capsids are isolated via ultracentrifugation, followed by chromatographic purification. The viral particles are analyzed with both molecular-based assays and transmission electron microscopy (TEM) for full viral vector characterizations, including titer quantitation and full-to-empty ratio. The payload inside the AAV reference materials is CMV-GFP, click to view the vector sequence.
The AAV standard materials (full capsids) are characterized for identity and purity, viral particles per milliliter (mL), vector genomes per mL, as well as full/empty ratio. The lot sizes range from a few thousand vials to tens of thousands of vials for each serotype of AAV standards.
TEM Analysis of AAV Reference Standards (Full Capsids)
Fig. 1. TEM (transmission electron microscope) image of purified AAV9 reference standards. Full capsids appear as white spheres (white arrow) while empty capsids appear as spheres with black dots in the center (black arrow),
Purity of AAV Reference Standards (Full Capsids) Analyzed With Silver Staining
Fig. 2. Silver staining of AAV reference standards (full capsids).
AAV reference standard materials were loaded on a Bis-Tris SDS-PAGE gel. Proteins were detected with silver staining.
Stability of AAV Reference Standards
The stability of AAV reference standards has been demonstrated with a separate stability study of rAAV at 3E+12 GC/mL. The AAV vectors were stored at -80oC for 2 years. Viral genome titer and total particle titer did not change, as assessed by qPCR and ELISA assays.
The stability of the reference standard (full capsids) will be conducted in an ongoing manner. qPCR test will be performed following storage at -80oC, at 3 months, 6 months, and yearly afterwards.
What Can the AAV Reference Standards (Full Capsids) be used for?
The well characterized AAV full capsids reference standards can be used for characterization of rAAV stocks and calibration of in-house reference standards.
- Reference standards for qPCR titer assays
- Controls/ Reference materials for full/empty characterization
- Bridging materials for in house reference standards
Need Custom AAV Reference Standards?
If you need a larger scale or a different serotype of AAV reference materials, we can provide custom production for you. The same production, purification procedure, and analytic methods will be used as the catalog AAV reference standards. Please contact us.