Explore the Fully-characterized AAV Reference Materials (Empty Capsids)
Serotype specific AAV empty particles are produced via transient transfection, purified using ultracentrifugation, followed with chromatographic purification. The empty viral particles are analyzed with both molecular-based assays and transmission electron microscopy (TEM) for full viral vector characterizations, including full-to-empty ratio.
The AAV standard materials (empty capsids) are characterized for purity, viral particles per mL, as well as full/empty ratio. A few thousands of vials per lot are available for each serotype of AAV reference material.
TEM Analysis of AAV Reference Material (Empty Capsids)
Fig.1. TEM (transmission electron microscope) image of purified AAV9 empty particles. Empty capsids appear as spheres with black dots in the center (black arrow).
Purity of AAV Reference Materials (Empty Capsids) Analyzed With Silver Staining
Fig. 2. Silver staining of AAV reference materials (empty capsids).
AAV reference materials were loaded on a Bis-Tris SDS-PAGE gel. Proteins were detected with silver staining.
Stability of AAV Reference Materials
The stability of AAV reference materials was demonstrated with a separate stability study of rAAV at 3E+12 GC/mL. The AAV vectors were stored at -80oC for 2 years. Viral genome titer and total particle titer did not change by qPCR and ELISA assays. The stability of the reference material (empty capsids) will be conducted in an ongoing manner. ELISA test will be performed following storage at -80oC, at 3 months, 6 months, and yearly afterwards.
What Can AAV Reference Materials (Empty Capsids) be used for?
Well characterized AAV empty materials can be used for characterization of rAAV stocks and as AAV controls.
- Standard materials for ELISA, HPLC assays
- Reference materials for in-house standard materials
- Control viral vectors
Need Custom AAV Materials?
If you need a larger scale or a different serotype of AAV reference materials, we can provide custom production for you. The same production procedure and analytical methods will be used as the catalog AAV reference materials. Please contact us.