Shuttle Vectors & Promoters

Shuttle Vectors & Promoters

Adenovirus Shuttle Vector

Our shuttle vectors and promoters can be purchased individually, combined with our compatible human ORF collection, or used for custom cloning projects.

See All Shuttle Vectors & Promoters >

Build your Custom Expression Vector

Our adenovirus shuttle vector and destination vectors can be purchased individually or combined with our compatible human ORF collection. If you wish to transfer an ORF into any of the backbones listed below, simply provide the following information when you request a quote:

  1. Catalog number or RefSeq of the ORF
  2. Catalog number or name of the desired vector backbone
  3. Desired customization/mutagenesis (optional)
  4. Packaging into viral particles (optional)

Adenovirus Shuttle Vector and Destination Vectors

Adenovirus Vector Promoter Reporter/Tag Selection
pENTER CMV FLAG, His Kan
pDEST-C-GFP CMV GFP Amp
pDEST-C-RFP CMV RFP Amp
pDEST-C-HA CMV HA Amp
pDEST-C-3XFLAG CMV 3XFLAG Amp
pDEST-C-Myc CMV Myc Amp
pDEST-N-His CMV His Amp
pDEST-N-FLAG CMV FLAG Amp
pDEST-N-Myc CMV Myc Amp
pDEST-N-HA CMV HA Amp
pDEST-N-GFP CMV GFP Amp
pDEST-N-RFP CMV RFP Amp
pDEST-EF1a-C-FLAG-His EF1a FLAG, His Amp
pDEST-PGK-C-FLAG-His PGK FLAG, His Amp

Lentivirus Destination Vectors

Lentivirus Vector Promoter Reporter/Tag Selection
pLenti-CMV-FLAG-His CMV FLAG, His Amp
pLenti-GFP-CMV CMV Myc, FLAG Amp
pLenti-Puro-CMV CMV Myc, FLAG Amp
pLenti-C-GFP CMV GFP Amp
pLenti-C-RFP CMV RFP Amp
pLenti-N-GFP CMV GFP Amp
pLenti-N-RFP CMV RFP Amp
pLenti-N-FLAG CMV FLAG Amp
pLenti-N-Myc CMV Myc Amp
pLenti-N-HA CMV HA Amp

AAV Promoters and Destination Vectors

AAV Vector Promoter Reporter/Tag Selection
pAV-FH CMV FLAG, His Amp
pAV-ALB ALB FLAG, His Amp
pAV-aMHC aMHC FLAG, His Amp
pAV-c-Fos c-Fos FLAG, His Amp
pAV-CAG CAG FLAG, His Amp
pAV-CaMKIIa CaMKIIa FLAG, His Amp
pAV-CK0.4 CK0.4 FLAG, His Amp
pAV-CK1.3 CK1.3 FLAG, His Amp
pAV-cTnT cTnT FLAG, His Amp
pAV-EF1a EF1a FLAG, His Amp
pAV-EFFS EFFS FLAG, His Amp
pAV-GFAP GFAP FLAG, His Amp
pAV-GFAP104 GFAP104 FLAG, His Amp
pAV-MBP MBP FLAG, His Amp
pAV-MCK MCK FLAG, His Amp
pAV-MeCP2 MeCP2 FLAG, His Amp
pAV-NSE NSE FLAG, His Amp
pAV-PDX1 PDX1 FLAG, His Amp
pAV-PGK PGK FLAG, His Amp
pAV-Rpe65 Rpe65 FLAG, His Amp
pAV-SST SST FLAG, His Amp
pAV-Syn Syn FLAG, His Amp
pAV-TBG TBG FLAG, His Amp
pAV-UBC UBC FLAG, His Amp
pAV-3xEnhancer MCK 3X enhancer MCK FLAG, His Amp

Need a Custom Product?

We also provide cloning services to allow you to fully customize your order. If you would like to request a custom order, please contact us with your project details or visit our Molecular Cloning page for more information.

Viral Packaging

Please contact us if you would like your vector packaged into viral particles for efficient gene delivery into a variety of dividing and non-dividing cell types. We offer viral packaging services ranging from small-scale packaging for in vitro work, to large-scale packaging for animal studies, all the way up to GMP production for use in clinical applications. Please visit our AAV, Adenovirus, or Lentivirus Packaging pages for more information.

plasmid sidepanel min

FAQ

How is an ORF shuttled into a different vector?
The ORF cloned into the pENTER adenovirus shuttle vector can be transferred into the other Vigene destination vectors through a simple "cut and paste" restriction enzyme digestion and ligation. A combination of two pairs of restriction enzymes, AsiSI/MluI (96%), AsiSI/RsrII (99%) covers 99.5% of all human cDNA ORFs. All human genes can be covered with a few additional restriction enzyme combinations, such as AsiSI/NotI, AscI/RsrII.
Are there any limitations to which ORFs can be shuttled?
If you want to generate viral particles, you must not exceed the packaging capacity of the virus: 7.5 kb for adenovirus, 6 kb for lentivirus, and 4.7 kb for AAV. Please note that the packaging capacity refers to the total insert size between the ITRs or LTRs (inclusively), including the promoter, ORF, reporter, tags, etc.
What is Vigene’s guarantee?
We guarantee that our adenovirus shuttle vector, all of our destination vectors, and our human cDNA clones will contain the sequences listed on their respective product description pages. Please note that we cannot guarantee protein overexpression because it will depend on your experimental conditions.

1 to 10 of 65 Items

Page
per page
Set Descending Direction
  1. pAV-3xEnhancer MCK
    PM10023
    pAV-3xEnhancer MCK
    3XenhancerMcK
    FLAG, His
  2. pAV-4in1-shRNA
    P100039
    pAV-4in1-shRNA
    H1, U6
    GFP
  3. pAV-ALB
    PM10001
    pAV-ALB
    ALB
    FLAG, His
  4. pAV-aMHC
    PM10012
    pAV-aMHC
    aMHC
    FLAG, His
  5. pAV-c-Fos
    PM10016
    pAV-c-Fos
    c-Fos
    FLAG, His
  6. pAV-CAG
    PM10003
    pAV-CAG
    CAG
    FLAG, His
  7. pAV-CaMKIIa
    PM10004
    pAV-CaMKIIa
    CaMKIIa
    FLAG, His
  8. pAV-CK0.4
    PM10007
    pAV-CK0.4
    CK0.4
    FLAG, His
  9. pAV-CK1.3
    PM10006
    pAV-CK1.3
    CK1.3
    FLAG, His
  10. pAV-CreOn-shRNA
    P100040
    pAV-CreOn-shRNA
    CMV
    mStrawberry, GFP

1 to 10 of 65 Items

Page
per page
Set Descending Direction

Our shuttle vectors and promoters can be purchased individually, combined with our compatible human ORF collection, or used for custom cloning projects.