Lentivirus Controls

Lentiviral Controls

Vigene offers lentivirus controls with a variety of promoters and tags. These lentivirus controls are ultra-purified and can be used to for in vitro or in vivo animal studies. Our lentivirus controls includes several cell/tissue-specific promoters and research practical reporters including Cre, GFP, luciferase, mCherry, and RFP.  All of Vigene’s lentivirus controls are available in three different titer levels.

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Explore Popular Lentivirus Controls

Name Promoter Reporter Titers Available
LV-EF1a-Cre EF1a Cre 1x10^7 IU/mL, 1x10^8 IU/mL, 1x10^9 IU/mL
LV-CMV-GFP CMV GFP 1x10^7 IU/mL, 1x10^8 IU/mL, 1x10^9 IU/mL
LV-EF1a-Luciferase EF1a Luciferase 1x10^7 IU/mL, 1x10^8 IU/mL, 1x10^9 IU/mL
LV-EF1a-mCherry EF1a mCherry 1x10^7 IU/mL, 1x10^8 IU/mL, 1x10^9 IU/mL
LV-EF1a-RFP EF1a RFP 1x10^7 IU/mL, 1x10^8 IU/mL, 1x10^9 IU/mL

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Why is ultracentrifugation purification important?

Purifying high-titer lentivirus controls at or above 1x10^7 IU/mL is important to ensure a high infection rate. Before the purification process, crude lysate lentivirus material may contain high levels of impurities which can affect the transduction efficiency. Vigene’s ultracentrifugation process saves time and resources for laboratories who are constrained by a lack of proper equipment or require immediate supplies for their research. The ultracentrifugation purified lentivirus can be used for in vitro cell culture and in vivo animal studies.

Vigene’s ultracentrifugation process

Lentivirus controls are purified using sucrose gradient ultracentrifugation to ensure safe in vitro or in vivo animal study research. Vigene’s suspension cell ultracentrifugation process increases the infectivity rate 50x per batch regardless of promoter or reporter. The viral titer is then measured using RT-qPCR and ready for application.

High Transduction Efficiency of the Ultra-purified CMV-GFP lentiviral Control

Fig. 1. Fluorescent image of CMV-GFP lentiviral control tranduced HEK293 cells. The ultracentrifugation purified CMV-GFP lentivirus controls were used to transduce HEK293 cells. The fluorescent image was taken 72 hours post transduction.

Need to a Custom Lentivirus Control?

If you do not see your desired control, please contact us with your project details. Our team of experienced scientists will work with you to design and generate the lentivirus control that best fits your needs. For more information about our cloning services, please visit our Molecular Cloning page.

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FAQ

What is Vigene’s guarantee?

Vigene guarantees that our lentivirus controls will contain the specified sequences detailed on their product description pages and specified viral titer. Please note that we cannot guarantee expression because it will depend on your experimental conditions.

5 Items

per page
Set Descending Direction
  1. Lentiviral Cre Control Particles, Ultracentrifugation Purified
    LV-EF1a-Cre
    pLenti-SV40-Puro-EF1a-Cre
    EF1a
    Cre
  2. Lentiviral GFP Control Particles, Ultracentrifugation Purified
    CV10002
    pLenti-CMV-GFP
    CMV
    GFP
  3. Lentiviral Luciferase Control Particles, Ultracentrifugation Purified
    LV-EF1a-Luciferase
    pLenti-SV40-Puro-EF1a-Luciferase
    EF1a
    Luciferase
  4. Lentiviral mCherry Control Particles, Ultracentrifugation Purified
    LV-EF1a-mCherry
    pLenti-SV40-Puro-EF1a-mCherry
    EF1a
    mCherry
  5. Lentiviral RFP Control Particles, Ultracentrifugation Purified
    LV-EF1a-RFP
    pLenti-SV40-Puro-EF1a-RFP
    EF1a
    RFP

5 Items

per page
Set Descending Direction

Vigene offers lentivirus controls with a variety of promoters and tags. These lentivirus controls are ultra-purified and can be used to for in vitro or in vivo animal studies. Our lentivirus controls includes several cell/tissue-specific promoters and research practical reporters including Cre, GFP, luciferase, mCherry, and RFP.  All of Vigene’s lentivirus controls are available in three different titer levels.