Explore Popular Lentivirus Controls
|LV-EF1a-Cre||EF1a||Cre||1x10^7 IU/mL, 1x10^8 IU/mL, 1x10^9 IU/mL|
|LV-CMV-GFP||CMV||GFP||1x10^7 IU/mL, 1x10^8 IU/mL, 1x10^9 IU/mL|
|LV-EF1a-Luciferase||EF1a||Luciferase||1x10^7 IU/mL, 1x10^8 IU/mL, 1x10^9 IU/mL|
|LV-EF1a-mCherry||EF1a||mCherry||1x10^7 IU/mL, 1x10^8 IU/mL, 1x10^9 IU/mL|
|LV-EF1a-RFP||EF1a||RFP||1x10^7 IU/mL, 1x10^8 IU/mL, 1x10^9 IU/mL|
Why is ultracentrifugation purification important?
Purifying high-titer lentivirus controls at or above 1x10^7 IU/mL is important to ensure a high infection rate. Before the purification process, crude lysate lentivirus material may contain high levels of impurities which can affect the transduction efficiency. Vigene’s ultracentrifugation process saves time and resources for laboratories who are constrained by a lack of proper equipment or require immediate supplies for their research. The ultracentrifugation purified lentivirus can be used for in vitro cell culture and in vivo animal studies.
Vigene’s ultracentrifugation process
Lentivirus controls are purified using sucrose gradient ultracentrifugation to ensure safe in vitro or in vivo animal study research. Vigene’s suspension cell ultracentrifugation process increases the infectivity rate 50x per batch regardless of promoter or reporter. The viral titer is then measured using RT-qPCR and ready for application.
High Transduction Efficiency of the Ultra-purified CMV-GFP lentiviral Control
Fig. 1. Fluorescent image of CMV-GFP lentiviral control tranduced HEK293 cells. The ultracentrifugation purified CMV-GFP lentivirus controls were used to transduce HEK293 cells. The fluorescent image was taken 72 hours post transduction.
Need to a Custom Lentivirus Control?
If you do not see your desired control, please contact us with your project details. Our team of experienced scientists will work with you to design and generate the lentivirus control that best fits your needs. For more information about our cloning services, please visit our Molecular Cloning page.
Viral Delivery Guide
Our Viral Delivery Guide will help you choose which virus will work best for your specific applications.
These protocols provide suggested guidelines for using lentivirus for in vitro and in vivo experiments.