Explore Popular GFP AAV Controls
|AAV GFP Testing Kit||CMV||GFP||Various|
How do I Know Which AAV Control to Use?
There are three major variables to consider when choosing an appropriate control: the promoter, the reporter, and the serotype. The promoter should ideally match that of the virus expressing your GOI. However, you have some flexibility with the reporter you choose as it will depend on your experimental conditions. Many scientists use fluorescent protein reporters, such as GFP AAV or RFP AAV, for their ease of use and detection. Lastly, the AAV serotype should match the tissue where your GOI is expressed. If you are unsure which promoter or serotype will work best for your experiments, please see the FAQ section at the bottom of this page.
What AAV Controls Are Available?
Please see the table below for a full list of the genetic elements that we have available in a variety of combinations and packaged in different AAV serotypes.
Note: When browsing our large AAV controls catalog, it is helpful to search by name or filter by promoter, reporter, and/or serotype to narrow down the search results.
|Promoters||ALB, aMHC, c-Fos, CAG, CaMKIIa, CK0.4, CK1.3, CMV, cTnT, EF1a, EFFS, GFAP, HCRApoE, MBP, MCK, MeCP2, NSE, PDX1, PGK, Rpe65, SST, Syn, TBG, UBC|
|Reporters||GFP, mCherry, RFP, LacZ, Luciferase|
|Inducible reporters||DIO-GFP, DIO-mCherry, DIO-RFP, DIO-LacZ|
|Inducible systems||Cre, DIO, tTA|
|CRISPR/Cas9||Cas9, gRNA scaffold|
|Serotypes||AAV1, AAV2, AAV5, AAV6, AAV7, AAV8, AAV9, AAV-DJ|
Need a Custom AAV Control?
If you do not see your desired control, please contact us with your project details. Our team of experienced scientists will work with you to design and generate the AAV control that best fits your needs. For more information about our cloning services, please visit our Molecular Cloning page.
Viral Delivery Guide
Our Viral Delivery Guide will help you choose which virus will work best for your specific applications.
These protocols provide suggested guidelines for using AAV for in vitro and in vivo experiments.
What is Vigene’s guarantee?
Which promoter should I use?
Please use this table to help you decide which promoter may work best for your experiments.
|Cell/Tissue type||Recommended Promoters|
|Neurons||CaMKIIa, CK0.4, CK1.3, Syn, MeCP2, c-Fos, NSE, SST|
|Ubiquitous||CAG, CMV, EF1a, EFFS, UBC, PGK|
Which serotype should I use?
Specificity is determined by the viral capsid proteins, so it is important to select the correct AAV serotype to ensure optimal gene delivery. The table below lists our recommended AAV serotypes for different target tissues. If you are unsure which AAV serotype will work best for your system, we recommend that you try out our AAV GFP Testing Kit. The testing kit contains the following viruses all driven by the strong CMV promoter: GFP AAV1, GFP AAV2, GFP AAV5, GFP AAV6, GFP AAV8, GFP AAV9, and GFP AAV-DJ.
|Tissue type||Recommended AAV Serotypes|
|Muscle||AAV1, AAV6, AAV8, AAV9|
|Liver||AAV8, AAV9, AAV-DJ|
|Lung||AAV5, AAV6, AAV9|
|Central Nervous System||AAV1, AAV5, AAV8, AAV9, AAV-DJ|
|Retina||AAV1, AAV2, AAV5, AAV8|
|Heart||AAV1, AAV8, AAV9|