Explore AAV Biosensors
Vigene’s biosensors come with the choice of promoter (CMV or Syn), serotype (AAV8 or AAV9), and many have an optional DIO/FLEX system for Cre-inducible expression. Please click on any of the biosensor names below for a list of our available products. Please see our FAQs section or Biosensors Handbook at the bottom of the page for more information.
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What Are Biosensors?
Biosensors are genetically engineered fluorescent proteins (FP) attached to additional protein sequences that makes them sensitive to small biomolecules or other physiological intracellular processes (e.g., calcium). These biosensors are introduced into cells, tissues, or organisms to detect changes in fluorescence intensity or spectrum. Many biosensors permit long-term imaging and can be engineered to specifically target cellular compartments or organelles. Additionally, biosensors permit signaling pathway exploration or allow for the measurement of a biomolecule. They do all this while principally preserving both spatial as well as temporal cellular processes.
How Do Biosensors Work?
GCaMP biosensors and other similar biosensors are constructed using circularly-permuted fluorescent proteins (GFP in the case of GCaMPs). The native N- and C- termini are linked together and new termini are created in the middle of the protein in close proximity to the phenolate oxygen in the chromophore. A segment of the calcium-binding protein calmodulin (CaM) and its target peptide (M13) are individually fused to the new termini. In the presence of calcium, CaM binds to M13, which induces a conformational change that leads to increased fluorescence.
Viral Delivery Guide
Our Viral Delivery Guide will help you choose which virus will work best for your specific applications.
AAV Biosensors Handbook
Our AAV Biosensors Handbook gives an overview of the biosensors we offer and how to choose the right biosensor for your applications.
The plasmids were generated by the Douglas Kim lab. For more information about the jGCaMP7 variants, please see their publication.
Vigene guarantees that our AAV biosensors contain the specified sequences. However, we can cannot guarantee expression because it will depend on your experimental conditions.
AAV Biosensors are covered under US Patents #14/350,199; #8,629,256, #14/800,814, #14/800,814, #14/941,406, #14/974,483, 14/941,406, 14/974,483, 14/452,428 and foreign equivalents and licensed from Janelia Research Campus, HHMI, Janelia, Virginia, USA. The products and the reagents generated from these services shall be used as tools for research purposes, and shall exclude any human or clinical use. The purchase of the AAV Biosensor Products conveys to the purchaser the limited, non-transferable right to use the products purchased and the reagents generated from Vigene Biosciences, Inc. services and any related material solely for Research Purposes only, not for any Commercial Purposes.