AAV Biosensors

AAV Biosensors

GCaMP and iGluSnFR AAV Biosensor

Vigene is proud to offer AAV calcium and glutamate biosensors made possible through a world-wide license from Janelia Research Campus. Our AAV biosensors are ideal for a variety of applications, such as tracking neuronal dynamics. We currently offer the following biosensors: CaMPARI, GCaMP, jRCaMP1, jRGECO1, and iGluSnFR. We have more biosensor products in our pipeline, so please check back periodically or contact us if you have a specific request.

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Explore AAV Biosensors

Vigene’s biosensors come with the choice of promoter (CMV or Syn), serotype (AAV8 or AAV9), and many have an optional DIO/FLEX system for Cre-inducible expression. Please click on any of the biosensor names below for a list of our available products. Please see our FAQs section or Biosensors Handbook at the bottom of the page for more information.

Type Fluorescence Biosensor Advantages
Calcium
Green to Red
  • Image total calcium activity during defined time windows gated by photoconversion light
  • Not restricted to microscope field of view, unlike real-time calcium imaging with GCaMP
  • Enables higher-throughput calcium assays with cultured cells
Calcium Green
  • Sensitivity, dynamics, and kinetics that can exceed synthetic indicators and show significant improvement over existing GECIs
  • Several variants to select from with overall brightness, rise/decay kinetics, and calcium affinity tuned for diverse applications
Calcium Red
  • Minimal bleed through into green channel
  • Combines improved sensitivity with photostability
  • Can be used for deep-tissue imaging, dual-color imaging with GFP-based reporters, and simultaneous use with ChR2
Calcium Red
  • Increased red fluorescence sensitivity, comparable to GCaMP6
  • Best performer for tracking sensitive stimuli
  • Can be used for deep-tissue imaging, dual-color imaging with GFP-based reporters, and simultaneous use with ChR2
Glutamate Green
  • Rapid detection of glutamate with high spatial resolution
  • Enables direct visualization of synaptic release
  • Improved SNR compared to existing glutamate sensors
  • Genetically-encoded and thus can be targeted to specific cellular populations and sub-cellular locations

What Are Biosensors?

Biosensors are genetically engineered fluorescent proteins (FP) attached to additional protein sequences that makes them sensitive to small biomolecules or other physiological intracellular processes (e.g., calcium). These biosensors are introduced into cells, tissues, or organisms to detect changes in fluorescence intensity or spectrum. Many biosensors permit long-term imaging and can be engineered to specifically target cellular compartments or organelles. Additionally, biosensors permit signaling pathway exploration or allow for the measurement of a biomolecule. They do all this while principally preserving both spatial as well as temporal cellular processes.

How Do Biosensors Work?

GCaMP biosensors and other similar biosensors are constructed using circularly-permuted fluorescent proteins (GFP in the case of GCaMPs). The native N- and C- termini are linked together and new termini are created in the middle of the protein in close proximity to the phenolate oxygen in the chromophore. A segment of the calcium-binding protein calmodulin (CaM) and its target peptide (M13) are individually fused to the new termini. In the presence of calcium, CaM binds to M13, which induces a conformational change that leads to increased fluorescence.

How GCaMP biosensors work

AAV sidepanel

FAQ

Where can I find more information about biosensors?
For more information, please visit the following Janelia pages about CaMPARI, GCaMP, jRCaMP1/jRGECO1, and iGluSnFR. You can also read our Biocompare article to learn why AAV biosensors are a powerful tool for tracking neuronal dynamics.
What are the differences between the different GCaMP biosensors?
The number following GCaMP refers to the generation (3rd, 5th, 6th, or 7th). The letter at the end refers to the characteristics of the variant.
jGCaMP7s: sensitive and slow
jGCaMP7f: fast kinetics
jGCaMP7b: brighter baseline
jGCaMP7c: high contrast

The plasmids were generated by the Douglas Kim lab. For more information about the jGCaMP7 variants, please see their publication.

What is Vigene’s guarantee?

Vigene guarantees that our AAV biosensors contain the specified sequences. However, we can cannot guarantee expression because it will depend on your experimental conditions.

Legal Statement

AAV Biosensors are covered under US Patents #14/350,199; #8,629,256, #14/800,814, #14/800,814, #14/941,406, #14/974,483, 14/941,406, 14/974,483, 14/452,428 and foreign equivalents and licensed from Janelia Research Campus, HHMI, Janelia, Virginia, USA. The products and the reagents generated from these services shall be used as tools for research purposes, and shall exclude any human or clinical use. The purchase of the AAV Biosensor Products conveys to the purchaser the limited, non-transferable right to use the products purchased and the reagents generated from Vigene Biosciences, Inc. services and any related material solely for Research Purposes only, not for any Commercial Purposes.

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  1. AAV8-CAG-FLEX-CaMPARI
    BS10-CXRAAV8
    pAAV-CAG-FLEX-CaMPARI
    CAG
    CaMPARI
  2. AAV8-CAG-FLEX-GCaMP6f-WPRE
    BS3-CXFAAV8
    pGP-AAV-CAG-FLEX-GCaMP6f-WPRE.25.693
    CAG
    GCaMP6f
  3. AAV8-CAG-FLEX-GCaMP6m-WPRE
    BS2-CXMAAV8
    pGP-AAV-CAG-FLEX-GCaMP6m-WPRE.25.629
    CAG
    GCaMP6m
  4. AAV8-CAG-FLEX-GCaMP6s-WPRE
    BS1-CXSAAV8
    pGP-AAV-CAG-FLEX-GCaMP6s-WPRE.25.641
    CAG
    GCaMP6s
  5. AAV8-CAG-FLEX-NES-jRCaMP1a-WPRE
    BS6-CXAAAV8
    pGP-AAV-CAG-FLEX-NES-jRCaMP1a-WPRE.216.1488
    CAG
    jRCaMP1a
  6. AAV8-CAG-FLEX-NES-jRCaMP1b-WPRE
    BS7-CXBAAV8
    pGP-AAV-CAG-FLEX-NES-jRCaMP1b-WPRE.216.1519
    CAG
    jRCaMP1b
  7. AAV8-CAG-FLEX-NES-jRGECO1a-WPRE
    BS8-CXAAAV8
    pGP-AAV-CAG-FLEX-NES-jRGECO1a-WPRE.116.1670
    CAG
    jRGECO1a
  8. AAV8-CAG-FLEX-NES-jRGECO1b-WPRE
    BS9-CXBAAV8
    pGP-AAV-CAG-FLEX-NES-jRGECO1b-WPRE.116.1721
    CAG
    jRGECO1b
  9. AAV8-CAG-GCaMP3
    BS4-CX3AAV8
    pAAV-CAG-GCaMP3
    CAG
    GCaMP3
  10. AAV8-CMV-iGluSnFR
    BS11-COG-AAV8
    pCMV(MinDis).iGluSnFR
    CMV
    iGluSnFR

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Vigene is proud to offer AAV calcium and glutamate biosensors made possible through a world-wide license from Janelia Research Campus. Our AAV biosensors are ideal for a variety of applications, such as tracking neuronal dynamics. We currently offer the following biosensors: CaMPARI, GCaMP, jRCaMP1, jRGECO1, and iGluSnFR. We have more biosensor products in our pipeline, so please check back periodically or contact us if you have a specific request.