Vigene Biosciences is unique in that we are one of the only companies that provides lentivirus services ranging from small-scale lentivirus packaging for in vitro work, to large-scale lentivirus packaging for animal studies, to lentivirus CGMP production for use in clinical applications. No matter what stage your research is at, our team of scientists will work with you to provide the custom lentivirus packaging service that best meets your needs.
Lentivirus Packaging Services
Below is a list of the lentivirus packaging services that we provide. We are also happy to customize your order, but please be aware that the prices and delivery times will vary based on the complexity of the order.
(for in vitro)
|Cell-culture Grade lentivirus packaging
1x107 IFU/mL, 200µL
(2 x 100µL)
(for in vitro or in vivo)
|Small-scale lentivirus packaging
100 µL (4 x 25 uL)
|Large-scale lentivirus packaging
200 µL (8 x 25 uL)
|Preclinical lentivirus packaging
||Total yield up to 1012 IFU
||Please inquire for price and estimated timeline
|CGMP lentivirus production
||Total yield up to 1012 IFU
||Please see our Lentivirus CGMP Production page
IFU = infectious units
*Note: The indicated titers can not be guaranteed if the fragment between the LTRs exceeds 6.0 kb. Please see the FAQ
section at the bottom of this page for more information.
Why Choose Lentivirus?
Lentiviruses are a subgroup of retroviruses: viruses that use viral reverse transcriptase to produce DNA from their RNA genomes before integrating into the host genome. Unlike other retroviruses, however, lentiviruses have the ability to infect both dividing and non-dividing cells. To increase safety, the components for virus replication are divided across multiple plasmids during recombinant lentivirus production. Lentivirus is a particularly useful tool for generating stable cell lines expressing your sequence of interest or for gene therapy applications such as CAR-T.
Note: Vigene offers only recombinant lentivirus products and services. We do not work with or sell wild-type lentivirus.
- Integrates into host genome
- Infects both dividing and non-dividing cells
- Strong and stable transgene expression
- Large packaging capacity (~9 kb)
- Can be used to make stable cell lines
- Potential for insertional mutagenesis due to random integration
- Titer is highly sensitive to viral genome size
- Small probability of generating replication-competent lentivirus
Comparison to Other Viruses
Please see our AAV and Adenovirus Packaging pages for more information.
Vigene Is Your Choice for Your Lentivirus Packaging Needs
There are several reasons why customers choose Vigene Biosciences for lentivirus packaging services. As an award-winning leader in viral vector manufacturing and development, we have built a strong track record of providing our customers with the high-quality materials they need to continue their work. At Vigene, it’s our mission to make the gene therapy process as affordable, customized, and straightforward as possible.
- Flexible scales – From small scale cell culture grade to ultrapure lentivirus for animal studies, to GMP lentivirus manufacturing for clinical applications.
- One stop lentiviral packaging service – From lentiviral vector custom designing, plasmid DNA preparation, to the final lentivirus packaging
- 3rd generation lentiviral vectors – Replication deficient lentivirus
- A variety of lentiviral vector backbones – CMV, EF1a, CAG, GFAP and more promoters, different cell selections and tags available
- Lentivirus experts work with you to provide lentiviruses that suit your need
Finally, we offer our customers the Vigene Guarantee. Your lentivirus packaging will contain your insert at the correct, designated titer based on the approved quote we provided you. If our first attempt doesn’t achieve the right titer, we’ll gladly perform the work again at no cost to you.
Recombinant Lentivirus Packaging
Recombinant lentiviruses are derived from HIV-1, so certain precautions must be taken to ensure its safe use in gene delivery. The biggest concern is the possibility of generating replication-competent lentivirus through crossover events between elements in the viral vector and the packaging cell line. To improve safety, viral accessory genes are deleted and essential genes are supplied in trans by the packaging and envelope plasmids. To produce lentivirus particles, the transfer plasmid containing the gene of interest (GOI), the envelope plasmid, and the packaging plasmid are co-transfected into the packaging cell line (usually HEK293T cells).
For our lentivirus packaging services, the lentivirus particles are purified using sucrose gradient ultracentrifugation to ensure safe use in vivo. Viral titer is then determined using RT-qPCR with primers to the long-inverted terminal repeats (LTRs) present in the viral genome. The viral particles are then shipped overnight and can be used immediately for in vitro or in vivo research.
Note: By default, our Lentivirus Packaging services use 2nd generation lentiviral plasmids. Please specify when requesting a quote if you require 3rd generation lentivirus packaging.
Biosafety of Recombinant Lentiviruses
Since lentivirus can infect human cells, precautions should be strictly followed. Recombinant lentiviruses are considered to be at Biosafety level 2 (BSL2). Safety considerations have been implemented when engineering recombinant lentiviral vectors as gene delivery tools. One major safety strategy is splitting the viral essential genes and LTR containing Lentiviral transfer vector into separate plasmids. The gene of interest (GOI) that is going to be delivered into cells is provided on a separate plasmid, called lentiviral transfer vector, which is flanked by the long terminal repeats (LTRs). The lentiviral transfer vector containing the GOI and LTRs will be packaged into the lentiviral particles, but the lentiviral packaging plasmids containing the viral essential genes will not be packaged due to lack of the packaging signal and LTRs. Vigene’s lentiviral vectors are 3rd generation vector, which is even safer. 5’ LTR is hybrid and the 3’ SIN (self inactivating) LTR does not support lentiviral replication after integrating into the host genome, which makes it to be replication deficient.
Generating Stable Cell Lines
In contrast to using transient transfection methods or other viruses, lentivirus can be used to generate stable cell lines expressing a gene of interest to study long-term protein expression. Lentiviral vectors randomly integrate their genetic cargo into the host genome (with a preference for actively transcribed genes) without transferring sequences that encode for proteins derived from the packaging virus. We offer lentiviral vectors which contain a puromycin-resistance gene that allows transduced cells to be selected for on the basis of puromycin resistance. In addition to our custom lentivirus packaging services, we have an extensive collection of ready-to-use human ORFs packaged into lentivirus.
We use a validated assay for measuring viral titer for all lentivirus packaging services. Titering is performed using RT-qPCR with primers targeting the long-inverted terminal repeat (LTR) present in the viral genome and quantified by comparison to a standard curve of a plasmid sample of known concentration. We then use a formula to convert to functional titer (IFU/mL). If you would like any additional quality control services added to your lentivirus packaging order, please see our Analytical Services page for more information.
Custom Lentivirus Vector Design
Vigene offers a broad range of custom lentivirus cloning services at a reasonable cost and with a quick turnaround time. Our highly experienced scientists are ready to tackle difficult cloning projects, including large or toxic clones or clones with high GC content or highly repetitive sequences. Please contact us with your project details and our team will get in touch as soon as possible with an estimated price and turnaround time.
Viral Delivery Guide
Our Viral Delivery Guide will help you choose which virus will work best for your specific applications.
Learn More >
These protocols provide suggested guidelines for using lentivirus for in vitro and in vivo experiments.
Learn More >
Research-grade vs. CGMP Brochure
Our brochure is designed to help researchers determine which grade of plasmid/virus is required at different stages of development.
Learn More >
Is recombinant lentivirus safe to use?
All work with lentiviruses should be performed in a BSL-2 facility, which is the biosafety level of most cell culture facilities. For all of our lentivirus packaging services, we use replication-incompetent lentivirus that cannot produce viral particles except in the presence of the packaging and envelope plasmids. Please note that although recombinant lentivirus is replication-incompetent, there is a very small chance of generating replication-competent lentivirus if recombination occurs between the packaging, envelope, and transfer plasmids and the resulting construct is packaged into a viral particle.
We primarily use 2nd generation lentiviral plasmids, which has the lentivirus genome edited down and distributed among three plasmids. We use 2nd generation because it is considered safe and produces higher viral titers than 3rd generation, which requires co-transfection with four plasmids. If you require 3rd generation, please specify while requesting a quote.
How and where should I send my plasmid?
Plasmids in TE buffer may be shipped at room temperature. Please check with us about shipping options if you use other buffers. We strongly recommend overnight shipping using an express carrier (e.g., UPS, FedEx) with tracking to minimize transit time.
Address your packages as follows:
Ref # [quote number]
5 Research Court
Rockville, MD, USA 20850
How much plasmid do I need to provide for lentivirus packaging?
If you will be ordering a clone you want packaged, please provide us with the RefSeq number or Vigene catalog number. If you will be sending us a plasmid, please refer to the table below. When possible, we recommend sending DNA obtained from a maxiprep. If you do not have the recommended amount of plasmid for lentivirus packaging, we can prepare endotoxin-free DNA from your sample for an additional fee.
||Amount of plasmid to send
||5-10 µg at conc. of >0.5 µg/µL
|Transformation (DNA preps)
|Small-scale lentivirus packaging
Titer: 2×108 IFU/mL
Large-scale lentivirus packaging
Titer: 109 IFU/mL
|>150 µg at 1 µg/µL or higher
>200 µg at 1 µg/µL or higher
What information is required for lentivirus packaging?
If you are sending a plasmid for lentivirus packaging, we require the following information to generate a quote:
- Plasmid name
- DNA concentration of plasmid (µg/µL)
- Size between the LTRs (including the LTRs)
- Antibiotic Resistance
- Sequence/map of plasmid (if available)
Note: Your project may be delayed if we do not receive sufficient information about your plasmid. You can provide this information when you request a quote.
What is Vigene’s guarantee?
Vigene guarantees that the packaged virus will contain the customer’s insert at the designated titer on the confirmed quote provided to the customer. If the designated titer is not achieved after one attempt, Vigene will repeat the lentivirus service a second time at no cost to the customer. If the designated titer is not obtained after the second attempt, we will contact the customer to discuss the project and potential options before proceeding. Please note that the titer for lentivirus is highly sensitive to viral genome size. Although the lentiviral packaging capacity is ~9 kb, we cannot guarantee viral titer if the LTR region is over 6 kb. Additional charges may be applied to achieve a requested titer under the circumstances described above.
Vigene offers one of the largest collections of human ORF clones packaged in lentivirus.
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Find the perfect control for your experiment from our catalog of lentivirus controls.
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More Details about Vigene's Lentivirus Packaging Services
Researchers depend on recombinant lentiviral vectors to achieve the most efficient gene delivery possible within mammalian cells. While plasmid DNA vectors can express genes in host cells, this expression is only episomal and transient. On the other hand, lentiviral vectors can permanently express foreign genes by integrating into the host genome, making it exceptionally powerful for both in vitro and in vivo research. No matter the size of your research project, Vigene offers you a wide range of lentivirus packaging services (from small to large-scale research) for a variety of different uses. We have many standard packaging options, but you can also customize your order with our team of scientists, allowing you to create the specific product you need for your research. Vigene provides several different grades of lentiviral vectors, allowing you to find the right product for your research needs. Crude (or unpurified) lentivirus is specifically intended for in vitro settings, while ultracentrifugation-purified lentivirus is ready for in vitro and in vivo animal studies. Finally, pre-clinical and clinical-grade CGMP lentivirus manufacturing is also available. Vigene offers fully integrated lentivirus packaging services. We can provide you with custom vector construction, plasmid preparation, and, finally, lentiviral vector production. We’ll help you through the entire lentivirus packaging process, making sure you get the materials you need without having to work through multiple vendors. That way, you can concentrate on your research instead of logistics. Whether your focus is research discoveries, clinical CGMP manufacturing, or scaling up to commercial manufacturing, Vigene is committed to ensuring you get access to the materials you need, all at a reasonable price.