Vigene Biosciences is unique in that we are one of the only companies that provides lentivirus services ranging from small-scale lentivirus packaging for in vitro work, to large-scale lentivirus packaging for animal studies, to lentivirus CGMP production for use in clinical applications. No matter what stage your research is at, our team of scientists will work with you to provide the custom lentivirus packaging service that best meets your needs.
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Biosafety of Recombinant Lentiviruses
Since lentivirus can infect human cells, precautions should be strictly followed. Recombinant lentiviruses are considered to be at Biosafety level 2 (BSL2). Safety considerations have been implemented when engineering recombinant lentiviral vectors as gene delivery tools. One major safety strategy is splitting the viral essential genes and LTR containing Lentiviral transfer vector into separate plasmids. The gene of interest (GOI) that is going to be delivered into cells is provided on a separate plasmid, called lentiviral transfer vector, which is flanked by the long terminal repeats (LTRs). The lentiviral transfer vector containing the GOI and LTRs will be packaged into the lentiviral particles, but the lentiviral packaging plasmids containing the viral essential genes will not be packaged due to lack of the packaging signal and LTRs. Vigene’s lentiviral vectors are 3rd generation vector, which is even safer. 5’ LTR is hybrid and the 3’ SIN (self inactivating) LTR does not support lentiviral replication after integrating into the host genome, which makes it to be replication deficient.
More Details about Vigene's Lentivirus Packaging Services
Researchers depend on recombinant lentiviral vectors to achieve the most efficient gene delivery possible within mammalian cells. While plasmid DNA vectors can express genes in host cells, this expression is only episomal and transient. On the other hand, lentiviral vectors can permanently express foreign genes by integrating into the host genome, making it exceptionally powerful for both in vitro and in vivo research. No matter the size of your research project, Vigene offers you a wide range of lentivirus packaging services (from small to large-scale research) for a variety of different uses. We have many standard packaging options, but you can also customize your order with our team of scientists, allowing you to create the specific product you need for your research. Vigene provides several different grades of lentiviral vectors, allowing you to find the right product for your research needs. Crude (or unpurified) lentivirus is specifically intended for in vitro settings, while ultracentrifugation-purified lentivirus is ready for in vitro and in vivo animal studies. Finally, pre-clinical and clinical-grade CGMP lentivirus manufacturing is also available. Vigene offers fully integrated lentivirus packaging services. We can provide you with custom vector construction, plasmid preparation, and, finally, lentiviral vector production. We’ll help you through the entire lentivirus packaging process, making sure you get the materials you need without having to work through multiple vendors. That way, you can concentrate on your research instead of logistics. Whether your focus is research discoveries, clinical CGMP manufacturing, or scaling up to commercial manufacturing, Vigene is committed to ensuring you get access to the materials you need, all at a reasonable price.